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Titre :
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Adhesin-specific nanomechanical cantilever biosensors for detection of microorganisms (2011)
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Auteurs :
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Tzuen-Rong J. Tzeng, Auteur ;
Yunyan R. Cheng, Auteur ;
Reza Saeidpourazar, Auteur
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Type de document :
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Article : texte imprimé
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Dans :
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Journal of heat transfer (Vol. 133 N° 1(N° Spécial), Janvier 2011)
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Article en page(s) :
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pp. [011012/1-5]
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Note générale :
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Physique
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Langues :
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Anglais
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Index. décimale :
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536 (Chaleur. Thermodynamique)
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Tags :
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Biosensors Cantilevers Microorganisms Molecular biophysics
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Résumé :
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Lectins (adhesins) on bacterial surfaces play important roles in infection by mediating bacterial adherence to host cell surfaces via their cognate receptors. We have explored the use of alpha-D-mannose receptors as capturing agents for the detection of Escherichia coli using a microcantilever and have demonstrated that E. coli ORN178, which expresses normal type-1 pili, can interact with microcantilevers functionalized with alpha-D-mannose and can cause shifts in its resonance frequencies. Although E. coli ORN208, which expresses abnormal pili, binds poorly to alpha-D-mannose on the nitrocellulose membrane of a FAST slide, it did cause a detectable shift in resonance frequency when interacting with the alpha-D-mannose functionalized microcantilevers.
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DEWEY :
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536
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ISSN :
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0022-1481
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En ligne :
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http://asmedl.aip.org/vsearch/servlet/VerityServlet?KEY=JHTRAO&ONLINE=YES&smode=strresults&sort=chron&maxdisp=25&threshold=0&pjournals=JHTRAO&pyears=2011&possible1zone=article&fromvolume=133&fromissue=1&OUTLOG=NO&sti=yes&viewabs=JHTRAO&key=DISPLAY&docID=7
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