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Laboratory evaluation of heterothalamus alienus essential oil against different pests of apis mellifera / Sergio R. Ruffinengo in The journal of essential oil research, Vol. 18 N° 6 (Novembre-Décembre 2006) 

Public ISBD [article]  in The journal of essential oil research > Vol. 18 N° 6 (Novembre-Décembre 2006) . - pp. 704-707 Titre : Laboratory evaluation of heterothalamus alienus essential oil against different pests of apis mellifera Type de document : texte imprimé Auteurs : Sergio R. Ruffinengo, Auteur ; Matías Maggi, Auteur ; Sandra Fuselli, Auteur Année de publication : 2006 Article en page(s) : pp. 704-707 Note générale : Génie Chimique Langues : Anglais (eng) Mots-clés : Heterothalamus  alienus  Asteraceae  Essential  oil  composition  β-pinene  Trans-muurola-4(14)  5-diene  Honeybee  pathogens  Ascosphaera  apis  Paenibacillus  larvae  Parasitic  bee  mite  Varroa  destructor Résumé : The Heterothalamus alienus oil was investigated in laboratory for control of different pests that affect the colonies of bees, Apis mellifera, against Varroa destructor mite, the bacterium that causes the American Foulbrood, Paenibacillus larvae and the fungus that produces the chalkbrood, Ascosphaera apis. The oil composition was analyzed by GC and GC/MS, the main components of the oil were β-pinene (44.4%) and trans-muurola-4(14),5-diene (9.2%). The concentration to kill 50% of the mites in 24 h (LC50) was 0.59 mg/cage. Inferior and superior limits were the following ones: 0.34 mg/cage and 1.01 mg/cage, LC50 was estimated for 48 h, and 72 h showed a slight increase with respect to the record of the 24 h. Paenibacillus larvae strains were Gram positive and catalase negative, the oil presented MIC values of 800–900 mg/L and MBC of 1000–1200 mg/L. Disks of impregnated flter paper with H. alienus oil around colonies of A. apis in growth inhibited the micelial growth signifcantly by 51% in the frst experiment (seven days) and by 31% in the second experiment (eight days). ISSN : 1041-2905 En ligne : http://www.tandfonline.com/doi/abs/10.1080/10412905.2006.9699211#.UXVNgkqC5mM [article] Laboratory evaluation of heterothalamus alienus essential oil against different pests of apis mellifera [texte imprimé] / Sergio R. Ruffinengo, Auteur ; Matías Maggi, Auteur ; Sandra Fuselli, Auteur . - 2006 . - pp. 704-707. Génie Chimique Langues : Anglais (eng) in The journal of essential oil research > Vol. 18 N° 6 (Novembre-Décembre 2006) . - pp. 704-707 Mots-clés : Heterothalamus  alienus  Asteraceae  Essential  oil  composition  β-pinene  Trans-muurola-4(14)  5-diene  Honeybee  pathogens  Ascosphaera  apis  Paenibacillus  larvae  Parasitic  bee  mite  Varroa  destructor Résumé : The Heterothalamus alienus oil was investigated in laboratory for control of different pests that affect the colonies of bees, Apis mellifera, against Varroa destructor mite, the bacterium that causes the American Foulbrood, Paenibacillus larvae and the fungus that produces the chalkbrood, Ascosphaera apis. The oil composition was analyzed by GC and GC/MS, the main components of the oil were β-pinene (44.4%) and trans-muurola-4(14),5-diene (9.2%). The concentration to kill 50% of the mites in 24 h (LC50) was 0.59 mg/cage. Inferior and superior limits were the following ones: 0.34 mg/cage and 1.01 mg/cage, LC50 was estimated for 48 h, and 72 h showed a slight increase with respect to the record of the 24 h. Paenibacillus larvae strains were Gram positive and catalase negative, the oil presented MIC values of 800–900 mg/L and MBC of 1000–1200 mg/L. Disks of impregnated flter paper with H. alienus oil around colonies of A. apis in growth inhibited the micelial growth signifcantly by 51% in the frst experiment (seven days) and by 31% in the second experiment (eight days). ISSN : 1041-2905 En ligne : http://www.tandfonline.com/doi/abs/10.1080/10412905.2006.9699211#.UXVNgkqC5mM

Laboratory evaluations of syzygium aromaticum (L.) merr. et perry essential oil against varroa destructor / Matias D. Maggi in The journal of essential oil research, Vol. 22 N° 2 (Mars/Avril 2010) 

Public ISBD [article]  in The journal of essential oil research > Vol. 22 N° 2 (Mars/Avril 2010) . - pp. 119-122 Titre : Laboratory evaluations of syzygium aromaticum (L.) merr. et perry essential oil against varroa destructor Type de document : texte imprimé Auteurs : Matias D. Maggi, Auteur ; Sergio R. Ruffinengo, Auteur ; Liesel B. Gende, Auteur Année de publication : 2010 Article en page(s) : pp. 119-122 Note générale : Génie Chimique Langues : Anglais (eng) Mots-clés : Syzygium  aromaticum  Myrtaceae  Essential  oil  composition  Eugenol  Mite  lethality  test  Apis  mellifera  Varroa  destructor Index. décimale : 646 Résumé : The oil obtained by hydrodistillation of the floral bottom of Syzygium aromaticum (L.) Merr. et Perry was analyzed by GC and GC/MS. Eugenol was the main constituent in the oil (86.7%). The biological activity of the oil applied to Varroa destructor and Apis mellifera was evaluated in two laboratory tests. Mite lethality was estimated using a complete exposure method test with the oil at different concentrations, and a systemic administration method of oil at different concentrations diluted in syrup was placed in feeders for bees. The LC50 for complete exposure method at 24 h was 0.59 mL/dish. The inferior and superior limits obtained were 0.47 x 10-6 mL/dish and 1.22 mL/dish, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection (LC50 of A. mellifera/ LC50 of V. destructor) for complete exposure method was 26.46 and 13.35 for 24 h and 48 h, respectively. Regarding the systemic administration method, mites LC50 at 24 h was 12,300 ppm. The inferior and superior limits calculated were 9,214 ppm and 15,178 ppm, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection for systemic administration method was 3.05 and 2.22 for 24 h and 48 h, respectively. Syzygium aromaticum oil was found to be an attractant for V. destructor at 4.8% (w/w) concentration. The results showed that oil toxicity against V. destructor differed depending upon its administration. Nevertheless, the ratio selection calculated by this oil is expected to enable its application under field conditions with a good safety margin. This oil could also be used in combination with other oils in integrated pest management strategies in bee colonies. DEWEY : 665 ISSN : 1041-2905 En ligne : http://www.jeoronline.com/archive/detail/50 [article] Laboratory evaluations of syzygium aromaticum (L.) merr. et perry essential oil against varroa destructor [texte imprimé] / Matias D. Maggi, Auteur ; Sergio R. Ruffinengo, Auteur ; Liesel B. Gende, Auteur . - 2010 . - pp. 119-122. Génie Chimique Langues : Anglais (eng) in The journal of essential oil research > Vol. 22 N° 2 (Mars/Avril 2010) . - pp. 119-122 Mots-clés : Syzygium  aromaticum  Myrtaceae  Essential  oil  composition  Eugenol  Mite  lethality  test  Apis  mellifera  Varroa  destructor Index. décimale : 646 Résumé : The oil obtained by hydrodistillation of the floral bottom of Syzygium aromaticum (L.) Merr. et Perry was analyzed by GC and GC/MS. Eugenol was the main constituent in the oil (86.7%). The biological activity of the oil applied to Varroa destructor and Apis mellifera was evaluated in two laboratory tests. Mite lethality was estimated using a complete exposure method test with the oil at different concentrations, and a systemic administration method of oil at different concentrations diluted in syrup was placed in feeders for bees. The LC50 for complete exposure method at 24 h was 0.59 mL/dish. The inferior and superior limits obtained were 0.47 x 10-6 mL/dish and 1.22 mL/dish, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection (LC50 of A. mellifera/ LC50 of V. destructor) for complete exposure method was 26.46 and 13.35 for 24 h and 48 h, respectively. Regarding the systemic administration method, mites LC50 at 24 h was 12,300 ppm. The inferior and superior limits calculated were 9,214 ppm and 15,178 ppm, respectively. LC50 estimated at 48 h showed a slight decrease as compared to that recorded at 24 h. Ratio selection for systemic administration method was 3.05 and 2.22 for 24 h and 48 h, respectively. Syzygium aromaticum oil was found to be an attractant for V. destructor at 4.8% (w/w) concentration. The results showed that oil toxicity against V. destructor differed depending upon its administration. Nevertheless, the ratio selection calculated by this oil is expected to enable its application under field conditions with a good safety margin. This oil could also be used in combination with other oils in integrated pest management strategies in bee colonies. DEWEY : 665 ISSN : 1041-2905 En ligne : http://www.jeoronline.com/archive/detail/50