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Adhesin-specific nanomechanical cantilever biosensors for detection of microorganisms / Tzuen-Rong J. Tzeng in Journal of heat transfer, Vol. 133 N° 1(N° Spécial) (Janvier 2011) 

Public ISBD [article]  in Journal of heat transfer > Vol. 133 N° 1(N° Spécial) (Janvier 2011) . - pp. [011012/1-5] Titre : Adhesin-specific nanomechanical cantilever biosensors for detection of microorganisms Type de document : texte imprimé Auteurs : Tzuen-Rong J. Tzeng, Auteur ; Yunyan R. Cheng, Auteur ; Reza Saeidpourazar, Auteur Année de publication : 2011 Article en page(s) : pp. [011012/1-5] Note générale : Physique Langues : Anglais (eng) Mots-clés : Biosensors  Cantilevers  Microorganisms  Molecular  biophysics Index. décimale : 536 Chaleur. Thermodynamique Résumé : Lectins (adhesins) on bacterial surfaces play important roles in infection by mediating bacterial adherence to host cell surfaces via their cognate receptors. We have explored the use of alpha-D-mannose receptors as capturing agents for the detection of Escherichia coli using a microcantilever and have demonstrated that E. coli ORN178, which expresses normal type-1 pili, can interact with microcantilevers functionalized with alpha-D-mannose and can cause shifts in its resonance frequencies. Although E. coli ORN208, which expresses abnormal pili, binds poorly to alpha-D-mannose on the nitrocellulose membrane of a FAST slide, it did cause a detectable shift in resonance frequency when interacting with the alpha-D-mannose functionalized microcantilevers. DEWEY : 536 ISSN : 0022-1481 En ligne : http://asmedl.aip.org/vsearch/servlet/VerityServlet?KEY=JHTRAO&ONLINE=YES&smode= [...] [article] Adhesin-specific nanomechanical cantilever biosensors for detection of microorganisms [texte imprimé] / Tzuen-Rong J. Tzeng, Auteur ; Yunyan R. Cheng, Auteur ; Reza Saeidpourazar, Auteur . - 2011 . - pp. [011012/1-5]. Physique Langues : Anglais (eng) in Journal of heat transfer > Vol. 133 N° 1(N° Spécial) (Janvier 2011) . - pp. [011012/1-5] Mots-clés : Biosensors  Cantilevers  Microorganisms  Molecular  biophysics Index. décimale : 536 Chaleur. Thermodynamique Résumé : Lectins (adhesins) on bacterial surfaces play important roles in infection by mediating bacterial adherence to host cell surfaces via their cognate receptors. We have explored the use of alpha-D-mannose receptors as capturing agents for the detection of Escherichia coli using a microcantilever and have demonstrated that E. coli ORN178, which expresses normal type-1 pili, can interact with microcantilevers functionalized with alpha-D-mannose and can cause shifts in its resonance frequencies. Although E. coli ORN208, which expresses abnormal pili, binds poorly to alpha-D-mannose on the nitrocellulose membrane of a FAST slide, it did cause a detectable shift in resonance frequency when interacting with the alpha-D-mannose functionalized microcantilevers. DEWEY : 536 ISSN : 0022-1481 En ligne : http://asmedl.aip.org/vsearch/servlet/VerityServlet?KEY=JHTRAO&ONLINE=YES&smode= [...]