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Design of protein crystallization processes guided by phase diagrams / Sze Kee Tam in Industrial & engineering chemistry research, Vol. 50 N° 13 (Juillet 2011) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 50 N° 13 (Juillet 2011) . - pp. 8163-8175 Titre : Design of protein crystallization processes guided by phase diagrams Type de document : texte imprimé Auteurs : Sze Kee Tam, Auteur ; Hok Chung Chan, Auteur ; Ka Ming Ng, Auteur Année de publication : 2011 Article en page(s) : pp. 8163-8175 Note générale : Chimie industrielle Langues : Anglais (eng) Mots-clés : Phase  diagram  Crystallization  Design Résumé : Many proteins including pharmaceuticals and industrial enzymes, after separation and purification, are high-value-added products. In this study, a general framework based on solid liquid equilibrium (SLE) phase diagrams for the conceptual design of protein crystallization processes is proposed. First, the coordinates for phase diagrams involving proteins are defined. Second, relevant regions of the phase diagram are determined by experiments. Finally, process synthesis is performed by visualizing the process paths on the phase diagram. This approach is demonstrated with two case studies. One is the design of a lysozyme recovery process in which salt-lean lysozyme solids are recovered from a lysozyme—sodium chloride solution. The other is the design of a process for separating lysozyme and ovalbumin in the presence of ammonium sulfate. The former design is in agreement with patent literature, and the latter design was verified with experiments. Thus, the feasibility of designing protein crystallization processes based on a phase diagram with ionic coordinates and color mapping is demonstrated. DEWEY : 660 ISSN : 0888-5885 En ligne : http://cat.inist.fr/?aModele=afficheN&cpsidt=24332141 [article] Design of protein crystallization processes guided by phase diagrams [texte imprimé] / Sze Kee Tam, Auteur ; Hok Chung Chan, Auteur ; Ka Ming Ng, Auteur . - 2011 . - pp. 8163-8175. Chimie industrielle Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 50 N° 13 (Juillet 2011) . - pp. 8163-8175 Mots-clés : Phase  diagram  Crystallization  Design Résumé : Many proteins including pharmaceuticals and industrial enzymes, after separation and purification, are high-value-added products. In this study, a general framework based on solid liquid equilibrium (SLE) phase diagrams for the conceptual design of protein crystallization processes is proposed. First, the coordinates for phase diagrams involving proteins are defined. Second, relevant regions of the phase diagram are determined by experiments. Finally, process synthesis is performed by visualizing the process paths on the phase diagram. This approach is demonstrated with two case studies. One is the design of a lysozyme recovery process in which salt-lean lysozyme solids are recovered from a lysozyme—sodium chloride solution. The other is the design of a process for separating lysozyme and ovalbumin in the presence of ammonium sulfate. The former design is in agreement with patent literature, and the latter design was verified with experiments. Thus, the feasibility of designing protein crystallization processes based on a phase diagram with ionic coordinates and color mapping is demonstrated. DEWEY : 660 ISSN : 0888-5885 En ligne : http://cat.inist.fr/?aModele=afficheN&cpsidt=24332141