| Titre : | Turnip (brassica rapa) peroxidase : purification and characterization (2010) |
| Auteurs : | Sepideh Motamed, Auteur ; Farnoosh Ghaemmaghami, Auteur ; Alemzadeh, Iran, Auteur |
| Type de document : | Article : texte imprimé |
| Dans : | Industrial & engineering chemistry research (Vol. 48 N° 23, Décembre 2009) |
| Article en page(s) : | pp. 10614–10618 |
| Note générale : | Industrial chemistry |
| Langues : | Anglais |
| Tags : | Turnip--(Brassica rapa)--Peroxidase--Purification--Characterization |
| Résumé : | Partial purification of plant peroxidase from turnip (Brassica rapa) was optimized. Aqueous two phase system and precipitation by ammonium sulfate (as two parallel purification methods) were used. Polyethylene glycol/ammonium sulfate/NaCl (25:7:3%, w/v) system followed by gel-filtration chromatography gave a purification factor of about 36 fold. On the other hand, ammonium sulfate precipitation (60−80% deg) followed by gel filtration gave only about 13 purification fold. Hence, the aqueous two-phase system was more efficient and useful method as a primary purification step since it was less laborious, less time-consuming, and led to more purification factor. The partially purified turnip enzyme was stable at a pH range 2.6−6.0 and had an optimum pH at 4.0. The enzyme was stable up to 55 °C with an optimum activity at 35 °C. |
| ISSN : | 0888-5885 |
| En ligne : | http://pubs.acs.org/doi/abs/10.1021/ie801997e |
