| Titre : | Preparation of mustard (Brassica juncea L.) protein isolate and recovery of phenolic compounds by ultrafiltration (2009) |
| Auteurs : | Ranjana Das, Auteur ; Chiranjib Bhattacharjee, Auteur ; Santinath Ghosh, Auteur |
| Type de document : | Article : texte imprimé |
| Dans : | Industrial & engineering chemistry research (Vol. 48 N° 10, Mai 2009) |
| Article en page(s) : | pp. 4939–4947 |
| Note générale : | Chemical engineering |
| Langues : | Anglais |
| Tags : | Mustard Ultrafiltration-diafiltration-based technique Phenolic antioxidants |
| Résumé : | Mustard (Brassica juncea L.) seed meal is good source of protein (28−36%) and phenolic antioxidants like sinapic acid and sinapine. In this work an ultrafiltration−diafiltration-based technique is developed for simultaneous preparation of protein isolate and recovery of phenolic rich fraction from seed meal using a high shear rotating disk membrane module with emphasis on reduced membrane fouling effect. The yield of membrane process found less (31.4% on dry basis) compared to precipitation process (42% on dry basis), but a high purity of protein isolate (96%) with improved functional properties was obtained. The phenolic content in the protein was found to be reduced from 7.23 mg/g of protein to as low as 0.6 mg/g of protein in this process. The phenolic-acid-rich fraction recovered shows about 87.24% radical scavenging and 34.3% chelation capacity per 100 ppm concentration. So, this process can be commercially exploited for protein isolate and phenolic antioxidant-rich product preparation after proper scale up. |
| En ligne : | http://pubs.acs.org/doi/abs/10.1021/ie801474q |
