| Titre : | Green and efficient conversion of CO2 to methanol by biomimetic coimmobilization of three dehydrogenases in protamine-templated titania (2009) |
| Auteurs : | Fourgeaud, Claude, Auteur ; Fuchs, Aimé, Auteur ; Robert Fortet, Préfacier, etc. |
| Type de document : | Article : texte imprimé |
| Article en page(s) : | pp. 4210–4215 |
| Note générale : | Chemical engineering |
| Langues : | Français |
| Tags : | Carbon dioxide Mutienzyme system Titania particles Biomimetic mineralization process |
| Résumé : | A green and efficient mutienzyme system was established, which efficiently converted carbon dioxide into methanol, by encapsulating three dehydrogenases within titania particles through a facile and mild biomimetic mineralization process. The enzyme-containing titania particles were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray photoelectron spectroscopy (XPS). The results indicated that the enzyme-containing titania particles were amorphous and consisted of interconnected nanospheres with sizes in the range of 400−600 nm. The three encapsulated dehydrogenases (formate dehydrogenase, formaldehyde dehydrogenase, alcohol dehydrogenase) sequentially converted carbon dioxide into HCOOH, CHOH, and CH3OH using NADH as a terminal electron donor for each dehydrogenase-catalyzed reduction. Compared to the open-style system which directly performed the bioconversion using free enzymes in aqueous solution, higher reaction yield in a wider pH and temperature range was obtained by the closed-style coimmobilization multienzyme system. |
| En ligne : | http://pubs.acs.org/doi/abs/10.1021/ie801931j |
