| Titre : | Microbial responses to surfactants |
| Auteurs : | Leila Laouar, Auteur ; K. C. Lowe, Directeur de thèse ; B. J. Mulligan, Directeur de thèse |
| Type de document : | texte imprimé |
| Editeur : | Nottingham : University of Nottingham, 1993 |
| Format : | 186 f. / ill. / 30 cm. |
| Note générale : |
Thèse de Doctorat : Génie Chimique : Angleterre, University of Nottingham : 1993
Annexe f. 187 - 226 . - Bibliogr. f. 227 - 248 |
| Langues : | Anglais |
| Index. décimale : | D000893 |
| Tags : | Pluronic F-68 Microbial cells Saccharomyces cerevisiae Triton X-100 Surfactants Alcohol dehydrogenase |
| Résumé : |
The effects of commercial grade pluronic F-68 or its purified fraction or Triton X-100 on growth of microbial cells in culture have been studied.
Growth of Saccharomyces cerevisiae, as determined by viable cell counts, was unaffected by culture with 0.1 - 10.0% (W/V) of commercial grade Pluronic F-68 or its purified fraction. However, cultures treated with 10.0% (W/V) commercial grade or purified Pluronic F-68 showed significabtly reduced absorbance up to the onset of the stationary phase of growth, as compared to controls. The responses to Pluronic F-68 varied according to concentration, age and purity of surfactant. Additionally, the storage temperature influenced the properties of Pluronic F-68. Growth in the presence of up to 10.0% (W/V) commercial or purified Pluronic F-68 had no effect on cell-associated protein concentration. However, a slight, but reproducible, increase in protein concentration was observed during the exponential/stationary phase in cultures supplemented with 0.1 - 1.0% Pluronic F-68. In addition, whole cell polypeptide profiles were unaffected by the presence of pluronic F-68. Triton X-100 altered growth kinetics, as reflected by an extended stationary phase and a slowing of the senescence phase. Additionally, in cultures supplemented with Triton, cell-associated protein concentration was proportional to cellular growth. Culture in the presence of Triton X-100 did not alter the polypeptide profiles characteristic of untreated cells at different phases of growth. The application of surfactants for cell permeabilization was also studied using both Pluronic F-68 and Triton X-100, together with Pluronic F-38 and pluronic L-35. Permeabilization was assayed by direct measurement of alcohol dehydrogenase (ADH) activity in whole cells. Mean ADH activity following incubation of S. cerevisiae with 0.2% (W/V or V/V) of either commercial or purified Pluronic F-68, Pluronic F-38, Pluronic L-35 or Triton X-100 for 30 min at 20°C was significantly greater than when cells were exposed to an identical concentration of the previously studied permeabilizing agent, cetyltrimethylammonium bromide (CTAB). |
Exemplaires (1)
| Cote | Support | Localisation | Section | Disponibilité | Spécialité | Etat_Exemplaire |
|---|---|---|---|---|---|---|
| D000893 | Papier + ressource électronique | Bibliothèque Annexe | Thèse de Doctorat | Disponible | Genie_chimique | Consultation sur place/Téléchargeable |
Documents numériques (1)
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LAOUAR.Leila.pdf URL
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