Chemical examination and evaluation of antioxidant and antimicrobial activities of essential oil from gymnema sylvestre R. Br. leaves / Dattatraya G. Naik in The journal of essential oil research, Vol. 23 N° 3 (Mai/Juin 2011)  

Public ISBD [article]  inThe journal of essential oil research > Vol. 23 N° 3 (Mai/Juin 2011) . - pp. 12-19 Titre : Chemical examination and evaluation of antioxidant and antimicrobial activities of essential oil from gymnema sylvestre R. Br. leaves Type de document : texte imprimé Auteurs : Dattatraya G. Naik, Auteur ; Chitra N. Dandge, Auteur ; Shobha V. Rupanar, Auteur Année de publication : 2011 Article en page(s) : pp. 12-19 Note générale : Génie Chimique Langues : Anglais (eng) Mots-clés : Antimicrobial activity Antioxidant assays Essential oil composition 4-ethyl guaiacol Eugenol Gymnema sylvestre R. Br. Hydroquinone Palmitic acid Total phenolic content 4-vinyl Index. décimale : 646 Résumé : The present study reports chemical examination and in vitro antioxidant and antimicrobial activities of essential oil isolated from leaves of a well known antidiabetic plant, Gymnema sylvestre R. Br. Essential oil was isolated from leaves by hydrodistillation using a Clevenger apparatus. The essential oil was found to contain 40 volatile compounds by GC/MS analysis. Hitherto unknown major components of essential oil identified by GC/MS analysis were found to be palmitic acid (17.8%), hydroquinone (13.9%), phytol (6.9%), pentadecanoic acid (6.8%), 4-vinyl guaiacol (4.5%), and eugenol (4.0%). Essential oil exhibited strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (IC50 28 μg/mL). Dose dependent antioxidant activity was shown by essential oil in β-carotene bleaching and 2,2-azinobis-(3-ethyl benzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging assays. The total phenolic content of essential oil was found to be 34 mg GAE/g fresh leaves. The essential oil inhibited the growth of Pseudomonas aeruginosa, Pseudomonas asplenii, Bacillus subtilis, Proteus mirabilis, Escherichia coli and fungus Candida albicans with minimal inhibitory concentration (MIC) varying between 22 mg/mL and 28 mg/mL. DEWEY : 665 ISSN : 1041-2905 En ligne : http://www.tandfonline.com/doi/abs/10.1080/10412905.2011.9700451 [article] Chemical examination and evaluation of antioxidant and antimicrobial activities of essential oil from gymnema sylvestre R. Br. leaves [texte imprimé] / Dattatraya G. Naik, Auteur ; Chitra N. Dandge, Auteur ; Shobha V. Rupanar, Auteur . - 2011 . - pp. 12-19. Génie Chimique Langues : Anglais (eng) in The journal of essential oil research > Vol. 23 N° 3 (Mai/Juin 2011) . - pp. 12-19 Mots-clés : Antimicrobial activity Antioxidant assays Essential oil composition 4-ethyl guaiacol Eugenol Gymnema sylvestre R. Br. Hydroquinone Palmitic acid Total phenolic content 4-vinyl Index. décimale : 646 Résumé : The present study reports chemical examination and in vitro antioxidant and antimicrobial activities of essential oil isolated from leaves of a well known antidiabetic plant, Gymnema sylvestre R. Br. Essential oil was isolated from leaves by hydrodistillation using a Clevenger apparatus. The essential oil was found to contain 40 volatile compounds by GC/MS analysis. Hitherto unknown major components of essential oil identified by GC/MS analysis were found to be palmitic acid (17.8%), hydroquinone (13.9%), phytol (6.9%), pentadecanoic acid (6.8%), 4-vinyl guaiacol (4.5%), and eugenol (4.0%). Essential oil exhibited strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (IC50 28 μg/mL). Dose dependent antioxidant activity was shown by essential oil in β-carotene bleaching and 2,2-azinobis-(3-ethyl benzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging assays. The total phenolic content of essential oil was found to be 34 mg GAE/g fresh leaves. The essential oil inhibited the growth of Pseudomonas aeruginosa, Pseudomonas asplenii, Bacillus subtilis, Proteus mirabilis, Escherichia coli and fungus Candida albicans with minimal inhibitory concentration (MIC) varying between 22 mg/mL and 28 mg/mL. DEWEY : 665 ISSN : 1041-2905 En ligne : http://www.tandfonline.com/doi/abs/10.1080/10412905.2011.9700451

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