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Molecular docking simulations for macromolecularly imprinted polymers / David R. Kryscio in Industrial & engineering chemistry research, Vol. 50 N° 24 (Décembre 2011) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 50 N° 24 (Décembre 2011) . - pp. 13877–13884 Titre : Molecular docking simulations for macromolecularly imprinted polymers Type de document : texte imprimé Auteurs : David R. Kryscio, Auteur ; Yue Shi, Auteur ; Pengyu Ren, Auteur Année de publication : 2012 Article en page(s) : pp. 13877–13884 Note générale : Chimie industrielle Langues : Anglais (eng) Mots-clés : Macromolecularly  Polymers Résumé : Molecularly imprinted polymers are fully synthetic antibody mimics prepared via the cross-linking of organic monomers in the presence of an analyte. This general procedure is now well developed for small-molecule templates; however, attempts to extend the same techniques to the macromolecular regime have achieved limited success to date. We employ molecular docking simulations to investigate the interactions between albumin, a common protein template, and frequently employed ligands used in the literature at the molecular level. Specifically, we determine the most favorable binding sites for these ligands on albumin and determine the types of noncovalent interactions taking place based on the amino acids present near this binding pocket. Our results show that hydrogen-bonding, electrostatic, and hydrophobic interactions occur between amino acid side chains and ligands. Several interactions are also taking place with the polypeptide backbone, potentially disrupting the protein’s secondary structure. We show that several of the ligands preferentially bind to the same sites on the protein, which indicates that if multiple monomers are used during synthesis, then competition for the same amino acids could lead to nonspecific recognition. Both of these results provide rational explanations for the lack of success to date in the field. DEWEY : 660 ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie201858n [article] Molecular docking simulations for macromolecularly imprinted polymers [texte imprimé] / David R. Kryscio, Auteur ; Yue Shi, Auteur ; Pengyu Ren, Auteur . - 2012 . - pp. 13877–13884. Chimie industrielle Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 50 N° 24 (Décembre 2011) . - pp. 13877–13884 Mots-clés : Macromolecularly  Polymers Résumé : Molecularly imprinted polymers are fully synthetic antibody mimics prepared via the cross-linking of organic monomers in the presence of an analyte. This general procedure is now well developed for small-molecule templates; however, attempts to extend the same techniques to the macromolecular regime have achieved limited success to date. We employ molecular docking simulations to investigate the interactions between albumin, a common protein template, and frequently employed ligands used in the literature at the molecular level. Specifically, we determine the most favorable binding sites for these ligands on albumin and determine the types of noncovalent interactions taking place based on the amino acids present near this binding pocket. Our results show that hydrogen-bonding, electrostatic, and hydrophobic interactions occur between amino acid side chains and ligands. Several interactions are also taking place with the polypeptide backbone, potentially disrupting the protein’s secondary structure. We show that several of the ligands preferentially bind to the same sites on the protein, which indicates that if multiple monomers are used during synthesis, then competition for the same amino acids could lead to nonspecific recognition. Both of these results provide rational explanations for the lack of success to date in the field. DEWEY : 660 ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie201858n