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Green synthesis of protein stabilized silver nanoparticles using pseudomonas fluorescens, a marine bacterium, and its biomedical applications when coated on polycaprolactam / Veluchamy Prabhawathi in Industrial & engineering chemistry research, Vol. 51 N° 14 (Avril 2012) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 51 N° 14 (Avril 2012) . - pp. 5230-5239 Titre : Green synthesis of protein stabilized silver nanoparticles using pseudomonas fluorescens, a marine bacterium, and its biomedical applications when coated on polycaprolactam Type de document : texte imprimé Auteurs : Veluchamy Prabhawathi, Auteur ; Ponnurengam Malliappan Sivakumar, Auteur ; Mukesh Doble, Auteur Année de publication : 2012 Article en page(s) : pp. 5230-5239 Note générale : Chimie industrielle Langues : Anglais (eng) Mots-clés : Bacteria  Pseudomonadales  Pseudomonadaceae  Pseudomonas Résumé : Green synthesis of protein stabilized silver nanoparticles (AgNPs) using the supernatant of a marine isolate, Pseudomonas fluorescens PMMD3 (P.fluorescens), and its biomedical applications and biocompatibility when coated on polycaprolactam is reported here. The AgNPs are spherical and are 1-10 nm in size. AgNP-coated polycaprolactam showed 89.7% and 92.4% reduction in colony forming units (CFUs), when compared to bare polymer, against S.aureus and E.coli, respectively. In the biofilm on AgNP composite, when compared to the bare polymer, there were carbohydrate reductions by factors of 3.5 and 6.0 in S.aureus and E.coli biofilm, respectively, and protein reductions by factors of 6.5 and 3.0 in S.aureus and E.coli biofilm, respectively. Reduction in the adhesion of S.aureus, C.albicans, A.niger, and F.proliferatum were observed using scanning electron miaoscopy (SEM) and fluorescence microscopy. The C.albicans biofilm is 15 and 6 μm thick on polycaprolactam and AgNP composite, respectively. We observed 84% and 80% proliferation of 3T3-L1 adipocyte cells and 85% and 97% of L929 fibroblast on the AgNP composite and bare polymer, indicating that this new material is suitable for biomaterial applications. ISSN : 0888-5885 En ligne : http://cat.inist.fr/?aModele=afficheN&cpsidt=25783432 [article] Green synthesis of protein stabilized silver nanoparticles using pseudomonas fluorescens, a marine bacterium, and its biomedical applications when coated on polycaprolactam [texte imprimé] / Veluchamy Prabhawathi, Auteur ; Ponnurengam Malliappan Sivakumar, Auteur ; Mukesh Doble, Auteur . - 2012 . - pp. 5230-5239. Chimie industrielle Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 51 N° 14 (Avril 2012) . - pp. 5230-5239 Mots-clés : Bacteria  Pseudomonadales  Pseudomonadaceae  Pseudomonas Résumé : Green synthesis of protein stabilized silver nanoparticles (AgNPs) using the supernatant of a marine isolate, Pseudomonas fluorescens PMMD3 (P.fluorescens), and its biomedical applications and biocompatibility when coated on polycaprolactam is reported here. The AgNPs are spherical and are 1-10 nm in size. AgNP-coated polycaprolactam showed 89.7% and 92.4% reduction in colony forming units (CFUs), when compared to bare polymer, against S.aureus and E.coli, respectively. In the biofilm on AgNP composite, when compared to the bare polymer, there were carbohydrate reductions by factors of 3.5 and 6.0 in S.aureus and E.coli biofilm, respectively, and protein reductions by factors of 6.5 and 3.0 in S.aureus and E.coli biofilm, respectively. Reduction in the adhesion of S.aureus, C.albicans, A.niger, and F.proliferatum were observed using scanning electron miaoscopy (SEM) and fluorescence microscopy. The C.albicans biofilm is 15 and 6 μm thick on polycaprolactam and AgNP composite, respectively. We observed 84% and 80% proliferation of 3T3-L1 adipocyte cells and 85% and 97% of L929 fibroblast on the AgNP composite and bare polymer, indicating that this new material is suitable for biomaterial applications. ISSN : 0888-5885 En ligne : http://cat.inist.fr/?aModele=afficheN&cpsidt=25783432