Extraction of Polyphenolic Compounds from Eucalyptus globulus Bark / Inês Mota in Industrial & engineering chemistry research, Vol. 51 N° 20 (Mai 2012)  

Public ISBD [article]  inIndustrial & engineering chemistry research > Vol. 51 N° 20 (Mai 2012) . - pp. 6991-7000 Titre : Extraction of Polyphenolic Compounds from Eucalyptus globulus Bark : Process Optimization and Screening for Biological Activity Type de document : texte imprimé Auteurs : Inês Mota, Auteur ; Paula C. Rodrigues Pinto, Auteur ; Catarina Novo, Auteur Année de publication : 2012 Article en page(s) : pp. 6991-7000 Note générale : Industrial chemistry Langues : Anglais (eng) Mots-clés : Biological activity Optimization Bark Résumé : The bark of Eucalyptus globulus is a promising source of polyphenolic compounds that could be extracted employing polar solvents. Extraction experiments were planned according to the Box-Behnken design to evaluate the effect of temperature and time of extraction and the ethanol/water ratio on the dependent variables total phenolic content (TPC; as gallic acid equivalents, GAE), antioxidant activity (AA; as millimoles of ascorbic acid equivalents, AAE), proanthocyanidins (Pac; as mimosa extract equivalents, MEE), Stiasny number (SN), and coextracted total carbohydrates (TC). Response surface models were developed, and statistical analysis of variance was performed. A maximum of TPC of 32% (wGAE/wextract) was achieved for 264 min, 82.5 °C, and 52% ethanol corresponding to about 2% of the bark weight. These conditions are close to those leading to maximum values of compounds with AA (2.1 mmolAAE/gextract) and Pac 14% (wMEE/wextract). TC in the extract and the SN predicted were 22.7% (wTC/wextract) and 37, respectively. Preliminary studies concerning the biological activity of some extracts have demonstrated their differential capacity to reduce human breast cancer cell proliferation. ISSN : 0888-5885 En ligne : http://cat.inist.fr/?aModele=afficheN&cpsidt=25925040 [article] Extraction of Polyphenolic Compounds from Eucalyptus globulus Bark : Process Optimization and Screening for Biological Activity [texte imprimé] / Inês Mota, Auteur ; Paula C. Rodrigues Pinto, Auteur ; Catarina Novo, Auteur . - 2012 . - pp. 6991-7000. Industrial chemistry Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 51 N° 20 (Mai 2012) . - pp. 6991-7000 Mots-clés : Biological activity Optimization Bark Résumé : The bark of Eucalyptus globulus is a promising source of polyphenolic compounds that could be extracted employing polar solvents. Extraction experiments were planned according to the Box-Behnken design to evaluate the effect of temperature and time of extraction and the ethanol/water ratio on the dependent variables total phenolic content (TPC; as gallic acid equivalents, GAE), antioxidant activity (AA; as millimoles of ascorbic acid equivalents, AAE), proanthocyanidins (Pac; as mimosa extract equivalents, MEE), Stiasny number (SN), and coextracted total carbohydrates (TC). Response surface models were developed, and statistical analysis of variance was performed. A maximum of TPC of 32% (wGAE/wextract) was achieved for 264 min, 82.5 °C, and 52% ethanol corresponding to about 2% of the bark weight. These conditions are close to those leading to maximum values of compounds with AA (2.1 mmolAAE/gextract) and Pac 14% (wMEE/wextract). TC in the extract and the SN predicted were 22.7% (wTC/wextract) and 37, respectively. Preliminary studies concerning the biological activity of some extracts have demonstrated their differential capacity to reduce human breast cancer cell proliferation. ISSN : 0888-5885 En ligne : http://cat.inist.fr/?aModele=afficheN&cpsidt=25925040

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