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Biomimetic synthesis of magnetic composite particles for laccase immobilization / Xiaobing Zheng in Industrial & engineering chemistry research, Vol. 51 N° 30 (Août 2012) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 51 N° 30 (Août 2012) . - pp. 10140–10146 Titre : Biomimetic synthesis of magnetic composite particles for laccase immobilization Type de document : texte imprimé Auteurs : Xiaobing Zheng, Auteur ; Qi Wang, Auteur ; Yanjun Jiang, Auteur Année de publication : 2012 Article en page(s) : pp. 10140–10146 Note générale : Industrial chemistry Langues : Anglais (eng) Mots-clés : Biomimetic  Magnetic  composite Résumé : Magnetic silica composite particles were prepared by using the biosilicification reaction, in which Fe3O4 nanoparticles were entrapped in a silica matrix. The composite particles were functionalized with 3-aminopropyltriethoxysilane (APTES). These functionalized magnetic composite particles were used to immobilize a kind of valuable bulky industrial enzyme (laccase). The incorporation of magnetic nanoparticles greatly facilitates the manipulation of the immobilized biocatalyst, since it can be easily and quickly recovered from the reaction system by the simple application of an external magnetic field. The effects of immobilization conditions were optimized and the activity of the immobilized laccase was investigated. The results showed that the highest specific activity of immobilized laccase reached to 224 U and the activity recovery was 83.9%. Compared with free laccase, the thermal, pH, operational and storage stabilities of the immobilized laccase were improved significantly. The catalytic activity of the immobilized laccase was also demonstrated by the degradation of two phenolic substances (i.e., 2,4-dichlorophenol and 4-chlorophenol). It was found that the removal rates of 2,4-dichlorophenol and 4-chlorophenol were 80.9% and 64.2% in about 12 h, respectively. ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie3000908 [article] Biomimetic synthesis of magnetic composite particles for laccase immobilization [texte imprimé] / Xiaobing Zheng, Auteur ; Qi Wang, Auteur ; Yanjun Jiang, Auteur . - 2012 . - pp. 10140–10146. Industrial chemistry Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 51 N° 30 (Août 2012) . - pp. 10140–10146 Mots-clés : Biomimetic  Magnetic  composite Résumé : Magnetic silica composite particles were prepared by using the biosilicification reaction, in which Fe3O4 nanoparticles were entrapped in a silica matrix. The composite particles were functionalized with 3-aminopropyltriethoxysilane (APTES). These functionalized magnetic composite particles were used to immobilize a kind of valuable bulky industrial enzyme (laccase). The incorporation of magnetic nanoparticles greatly facilitates the manipulation of the immobilized biocatalyst, since it can be easily and quickly recovered from the reaction system by the simple application of an external magnetic field. The effects of immobilization conditions were optimized and the activity of the immobilized laccase was investigated. The results showed that the highest specific activity of immobilized laccase reached to 224 U and the activity recovery was 83.9%. Compared with free laccase, the thermal, pH, operational and storage stabilities of the immobilized laccase were improved significantly. The catalytic activity of the immobilized laccase was also demonstrated by the degradation of two phenolic substances (i.e., 2,4-dichlorophenol and 4-chlorophenol). It was found that the removal rates of 2,4-dichlorophenol and 4-chlorophenol were 80.9% and 64.2% in about 12 h, respectively. ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie3000908