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Simultaneous purification of pulchinenoside B4 and B5 from pulsatilla chinensis using macroporous resin and preparative high performance liquid chromatography / Liming Wang in Industrial & engineering chemistry research, Vol. 51 N° 45 (Novembre 2012) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 51 N° 45 (Novembre 2012) . - pp. 14859-14866 Titre : Simultaneous purification of pulchinenoside B4 and B5 from pulsatilla chinensis using macroporous resin and preparative high performance liquid chromatography Type de document : texte imprimé Auteurs : Liming Wang, Auteur ; Qiongming Xu, Auteur ; Sheng Su, Auteur Année de publication : 2013 Article en page(s) : pp. 14859-14866 Note générale : Industrial chemistry Langues : Anglais (eng) Mots-clés : HPLC  chromatography  Macroporosity  Purification Résumé : Pulchinenoside B4 and B5 (PB4, PB5) are two major triterpenoid saponins existing in the roots of Pulsatilla chinensis (Bunge) Regel. In this study, a systematic preparative process was developed for the simultaneous purification of PB4 and PB5 from the herb. The performance and separation characteristics of nine types of macroporous resins were critically evaluated. Static absorption/desorption experiments revealed that LX17 belonging to the polyacrylate class possessed superior separation properties. Further dynamic absorption/desorption experiments on LX17 column were conducted to obtain the optimal parameters. To obtain both compounds with high purity, a second stage procedure was developed using preparative reversed-phase high performance liquid chromatography with a dynamic axial compression column system. The separation process was high-efficiency and low-cost, which indicated potential for industrial applications. ISSN : 0888-5885 En ligne : http://cat.inist.fr/?aModele=afficheN&cpsidt=26620399 [article] Simultaneous purification of pulchinenoside B4 and B5 from pulsatilla chinensis using macroporous resin and preparative high performance liquid chromatography [texte imprimé] / Liming Wang, Auteur ; Qiongming Xu, Auteur ; Sheng Su, Auteur . - 2013 . - pp. 14859-14866. Industrial chemistry Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 51 N° 45 (Novembre 2012) . - pp. 14859-14866 Mots-clés : HPLC  chromatography  Macroporosity  Purification Résumé : Pulchinenoside B4 and B5 (PB4, PB5) are two major triterpenoid saponins existing in the roots of Pulsatilla chinensis (Bunge) Regel. In this study, a systematic preparative process was developed for the simultaneous purification of PB4 and PB5 from the herb. The performance and separation characteristics of nine types of macroporous resins were critically evaluated. Static absorption/desorption experiments revealed that LX17 belonging to the polyacrylate class possessed superior separation properties. Further dynamic absorption/desorption experiments on LX17 column were conducted to obtain the optimal parameters. To obtain both compounds with high purity, a second stage procedure was developed using preparative reversed-phase high performance liquid chromatography with a dynamic axial compression column system. The separation process was high-efficiency and low-cost, which indicated potential for industrial applications. ISSN : 0888-5885 En ligne : http://cat.inist.fr/?aModele=afficheN&cpsidt=26620399