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Production of d-limonene in chitosan elicited citrus japonica suspension cultures / George Brian Lockwood in The journal of essential oil research, Vol. 19 N°2 (Mars-Avril 2007) 

Public ISBD [article]  in The journal of essential oil research > Vol. 19 N°2 (Mars-Avril 2007) . - pp. 113-116 Titre : Production of d-limonene in chitosan elicited citrus japonica suspension cultures Type de document : texte imprimé Auteurs : George Brian Lockwood, Auteur ; Supawan Bunrathep, Auteur ; Thanapat Songsak, Auteur Année de publication : 2007 Article en page(s) : pp. 113-116 Note générale : Génie Chimique Langues : Anglais (eng) Mots-clés : Citrus  japonica  Rutaceae  Chitosan  Elicitation  d-limonene  α-terpineol Résumé : Callus and cell suspension cultures of Citrus japonica Thunb. were established from seedlings. Chitosan was used as an elicitor for improving d-limonene content in cell suspension cultures. The effect of chitosan concentrations on cell growth and d-limonene content was studied. The optimum concentration of chitosan for d-limonene production by C. japonica Thunb. was 200 mg/L which, yielded 42.5 mg/L d-limonene at 12 days. Moreover, at 15 days, α-ter- pineol, a metabolite of d-limonene also reached the maximum content at 29.2 mg/L. At 21 days, both d-limonene and α-terpineol contents were 14.3 and 18.9 mg/L, respectively, which were increased 17 and 15 fold, respectively, higher than individual content without elicitation. ISSN : 1041-2905 En ligne : http://www.tandfonline.com/doi/abs/10.1080/10412905.2007.9699242 [article] Production of d-limonene in chitosan elicited citrus japonica suspension cultures [texte imprimé] / George Brian Lockwood, Auteur ; Supawan Bunrathep, Auteur ; Thanapat Songsak, Auteur . - 2007 . - pp. 113-116. Génie Chimique Langues : Anglais (eng) in The journal of essential oil research > Vol. 19 N°2 (Mars-Avril 2007) . - pp. 113-116 Mots-clés : Citrus  japonica  Rutaceae  Chitosan  Elicitation  d-limonene  α-terpineol Résumé : Callus and cell suspension cultures of Citrus japonica Thunb. were established from seedlings. Chitosan was used as an elicitor for improving d-limonene content in cell suspension cultures. The effect of chitosan concentrations on cell growth and d-limonene content was studied. The optimum concentration of chitosan for d-limonene production by C. japonica Thunb. was 200 mg/L which, yielded 42.5 mg/L d-limonene at 12 days. Moreover, at 15 days, α-ter- pineol, a metabolite of d-limonene also reached the maximum content at 29.2 mg/L. At 21 days, both d-limonene and α-terpineol contents were 14.3 and 18.9 mg/L, respectively, which were increased 17 and 15 fold, respectively, higher than individual content without elicitation. ISSN : 1041-2905 En ligne : http://www.tandfonline.com/doi/abs/10.1080/10412905.2007.9699242