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Monitoring cellulase protein adsorption and recovery using SDS-PAGE / Gang Hu in Industrial & engineering chemistry research, Vol. 49 N° 18 (Septembre 2010) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 49 N° 18 (Septembre 2010) . - pp. 8333–8338 Titre : Monitoring cellulase protein adsorption and recovery using SDS-PAGE Type de document : texte imprimé Auteurs : Gang Hu, Auteur ; John A. Heitmann, Auteur ; Orlando J. Rojas, Auteur Année de publication : 2010 Article en page(s) : pp. 8333–8338 Note générale : Industrial chemistry Langues : Anglais (eng) Mots-clés : Microcrystalline  celluloses Résumé : Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was employed to study the sorption behaviors of cellulases on microcrystalline celluloses and hardwood pulp. The adsorption and recovery of cellulases from Aspergillus niger and Trichoderma reesei were investigated at 25 °C. Cellulase recovery was conducted by rinsing adsorbed enzymes with sodium acetate buffer, Milli-Q water, and sodium hydroxide solution. The initial, equilibrium, and recovered enzymes were analyzed using SDS-PAGE gels. Gels were scanned and analyzed using ImagePro software. The molecular weights of cellulase proteins were determined using a protein marker having seven known proteins. The cellulase system from Trichoderma reesei had a higher adsorption on all substrates studied than the cellulase system from Aspergillius niger, and higher pH favored desorption from the substrates studied. Experimental results also demonstrated that adsorption and desorption amounts determined by SDS-PAGE were proportional to protein concentrations in their crude mixtures. ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie100731b [article] Monitoring cellulase protein adsorption and recovery using SDS-PAGE [texte imprimé] / Gang Hu, Auteur ; John A. Heitmann, Auteur ; Orlando J. Rojas, Auteur . - 2010 . - pp. 8333–8338. Industrial chemistry Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 49 N° 18 (Septembre 2010) . - pp. 8333–8338 Mots-clés : Microcrystalline  celluloses Résumé : Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was employed to study the sorption behaviors of cellulases on microcrystalline celluloses and hardwood pulp. The adsorption and recovery of cellulases from Aspergillus niger and Trichoderma reesei were investigated at 25 °C. Cellulase recovery was conducted by rinsing adsorbed enzymes with sodium acetate buffer, Milli-Q water, and sodium hydroxide solution. The initial, equilibrium, and recovered enzymes were analyzed using SDS-PAGE gels. Gels were scanned and analyzed using ImagePro software. The molecular weights of cellulase proteins were determined using a protein marker having seven known proteins. The cellulase system from Trichoderma reesei had a higher adsorption on all substrates studied than the cellulase system from Aspergillius niger, and higher pH favored desorption from the substrates studied. Experimental results also demonstrated that adsorption and desorption amounts determined by SDS-PAGE were proportional to protein concentrations in their crude mixtures. ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie100731b