Induction of sesquiterpenoid production by methyl jasmonate in aquilaria sinensis cell suspension culture / Michiho Ito in The journal of essential oil research, Vol. 17 N° 2 (Mars-Avril 2005)  

Public ISBD [article]  inThe journal of essential oil research > Vol. 17 N° 2 (Mars-Avril 2005) . - pp. 175-180 Titre : Induction of sesquiterpenoid production by methyl jasmonate in aquilaria sinensis cell suspension culture Type de document : texte imprimé Auteurs : Michiho Ito, Auteur ; Ken-ichiro Okimoto, Auteur ; Toru Yagura, Auteur Année de publication : 2006 Article en page(s) : pp. 175-180 Note générale : Génie Chimique Langues : Anglais (eng) Mots-clés : Aquilaria sinensis Thymelaeaceae Agarwood Methyl jasmonate Solid phase micro-extraction Cell culture Ses-quiterpenoid production Résumé : A suspension cell culture was established from Aquilaria sinensis, a timber species producing fragrant woody material called “Agarwood.” Methyl jasmonate was added to this culture in order to induce production of fragrant compounds. Cells were harvested after seven days of incubation and subjected to solid phase micro-extraction to produce an extract that was analyzed by GC/MS. Three peaks appeared on GC and were identifi ed as α-guaiene, γ-guaiene (= 1(10),11-guaiadiene), and α-humulene based on their mass fragmentation patterns and 13C-NMR data. The production of the guaiene derivatives and α-humulene showed different kinetics, which may mean they are the products of different induced biosynthetic enzymes. ISSN : 1041-2905 En ligne : http://www.tandfonline.com/doi/abs/10.1080/10412905.2005.9698867 [article] Induction of sesquiterpenoid production by methyl jasmonate in aquilaria sinensis cell suspension culture [texte imprimé] / Michiho Ito, Auteur ; Ken-ichiro Okimoto, Auteur ; Toru Yagura, Auteur . - 2006 . - pp. 175-180. Génie Chimique Langues : Anglais (eng) in The journal of essential oil research > Vol. 17 N° 2 (Mars-Avril 2005) . - pp. 175-180 Mots-clés : Aquilaria sinensis Thymelaeaceae Agarwood Methyl jasmonate Solid phase micro-extraction Cell culture Ses-quiterpenoid production Résumé : A suspension cell culture was established from Aquilaria sinensis, a timber species producing fragrant woody material called “Agarwood.” Methyl jasmonate was added to this culture in order to induce production of fragrant compounds. Cells were harvested after seven days of incubation and subjected to solid phase micro-extraction to produce an extract that was analyzed by GC/MS. Three peaks appeared on GC and were identifi ed as α-guaiene, γ-guaiene (= 1(10),11-guaiadiene), and α-humulene based on their mass fragmentation patterns and 13C-NMR data. The production of the guaiene derivatives and α-humulene showed different kinetics, which may mean they are the products of different induced biosynthetic enzymes. ISSN : 1041-2905 En ligne : http://www.tandfonline.com/doi/abs/10.1080/10412905.2005.9698867

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