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Ion-exchange processing of fermentation media containing lactic acid and oligomeric saccharides / Beatriz Gullón in Industrial & engineering chemistry research, Vol. 49 N° 8 (Avril 2010) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 49 N° 8 (Avril 2010) . - pp. 3741–3750 Titre : Ion-exchange processing of fermentation media containing lactic acid and oligomeric saccharides Type de document : texte imprimé Auteurs : Beatriz Gullón, Auteur ; Jose Luis Alonso, Auteur ; J. C. Parajó, Auteur Année de publication : 2010 Article en page(s) : pp. 3741–3750 Note générale : Industrial Chemistry Langues : Anglais (eng) Mots-clés : Ion-Exchange  Fermentation  Lactic  Acid  Oligomeric  Saccharides Résumé : Ion-exchange processing was employed to separate oligomers and lactic acid (LA) present in fermentation broths derived from apple pomace supplemented with yeast extract (YE) or corn steep liquor (CSL) using Lactobacillus rhamnosus. Experiments were carried out with Amberlite IRA 96 or Amberlite IRA 400 anion-exchange resins. Fermentation media supplemented with YE enabled higher oligosaccharide recovery yields than CSL-supplemented ones, whereas Amberlite IRA 96 provided a better separation of oligomers and LA than Amberlite IRA 400. Starting from a YE-supplemented fermentation medium containing 60.5 g of LA/L and 33.9 g of oligomers/L, column separation with Amberlite IRA 96 led to an effluent during the loading stage, which contained 17.9 g of oligosaccharides/L with an oligosaccharide/LA mass ratio of 90/10 g/g, whereas the regeneration stage provided a solution containing 30 g of LA/L with a oligosaccharide/LA mass ratio of 8/92 g/g. Material balances showed that the regeneration effluent contained about 55% of the LA present in the feed stream, whereas only 2.1% was eluted jointly with oligomers during the loading phase. ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie901397k [article] Ion-exchange processing of fermentation media containing lactic acid and oligomeric saccharides [texte imprimé] / Beatriz Gullón, Auteur ; Jose Luis Alonso, Auteur ; J. C. Parajó, Auteur . - 2010 . - pp. 3741–3750. Industrial Chemistry Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 49 N° 8 (Avril 2010) . - pp. 3741–3750 Mots-clés : Ion-Exchange  Fermentation  Lactic  Acid  Oligomeric  Saccharides Résumé : Ion-exchange processing was employed to separate oligomers and lactic acid (LA) present in fermentation broths derived from apple pomace supplemented with yeast extract (YE) or corn steep liquor (CSL) using Lactobacillus rhamnosus. Experiments were carried out with Amberlite IRA 96 or Amberlite IRA 400 anion-exchange resins. Fermentation media supplemented with YE enabled higher oligosaccharide recovery yields than CSL-supplemented ones, whereas Amberlite IRA 96 provided a better separation of oligomers and LA than Amberlite IRA 400. Starting from a YE-supplemented fermentation medium containing 60.5 g of LA/L and 33.9 g of oligomers/L, column separation with Amberlite IRA 96 led to an effluent during the loading stage, which contained 17.9 g of oligosaccharides/L with an oligosaccharide/LA mass ratio of 90/10 g/g, whereas the regeneration stage provided a solution containing 30 g of LA/L with a oligosaccharide/LA mass ratio of 8/92 g/g. Material balances showed that the regeneration effluent contained about 55% of the LA present in the feed stream, whereas only 2.1% was eluted jointly with oligomers during the loading phase. ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie901397k