Turnip (brassica rapa) peroxidase / Sepideh Motamed in Industrial & engineering chemistry research, Vol. 48 N° 23 (Décembre 2009)  

Public ISBD [article]  inIndustrial & engineering chemistry research > Vol. 48 N° 23 (Décembre 2009) . - pp. 10614–10618 Titre : Turnip (brassica rapa) peroxidase : purification and characterization Type de document : texte imprimé Auteurs : Sepideh Motamed, Auteur ; Farnoosh Ghaemmaghami, Auteur ; Alemzadeh, Iran, Auteur Année de publication : 2010 Article en page(s) : pp. 10614–10618 Note générale : Industrial chemistry Langues : Anglais (eng) Mots-clés : Turnip--(Brassica rapa)--Peroxidase--Purification--Characterization Résumé : Partial purification of plant peroxidase from turnip (Brassica rapa) was optimized. Aqueous two phase system and precipitation by ammonium sulfate (as two parallel purification methods) were used. Polyethylene glycol/ammonium sulfate/NaCl (25:7:3%, w/v) system followed by gel-filtration chromatography gave a purification factor of about 36 fold. On the other hand, ammonium sulfate precipitation (60−80% deg) followed by gel filtration gave only about 13 purification fold. Hence, the aqueous two-phase system was more efficient and useful method as a primary purification step since it was less laborious, less time-consuming, and led to more purification factor. The partially purified turnip enzyme was stable at a pH range 2.6−6.0 and had an optimum pH at 4.0. The enzyme was stable up to 55 °C with an optimum activity at 35 °C. ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie801997e [article] Turnip (brassica rapa) peroxidase : purification and characterization [texte imprimé] / Sepideh Motamed, Auteur ; Farnoosh Ghaemmaghami, Auteur ; Alemzadeh, Iran, Auteur . - 2010 . - pp. 10614–10618. Industrial chemistry Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 48 N° 23 (Décembre 2009) . - pp. 10614–10618 Mots-clés : Turnip--(Brassica rapa)--Peroxidase--Purification--Characterization Résumé : Partial purification of plant peroxidase from turnip (Brassica rapa) was optimized. Aqueous two phase system and precipitation by ammonium sulfate (as two parallel purification methods) were used. Polyethylene glycol/ammonium sulfate/NaCl (25:7:3%, w/v) system followed by gel-filtration chromatography gave a purification factor of about 36 fold. On the other hand, ammonium sulfate precipitation (60−80% deg) followed by gel filtration gave only about 13 purification fold. Hence, the aqueous two-phase system was more efficient and useful method as a primary purification step since it was less laborious, less time-consuming, and led to more purification factor. The partially purified turnip enzyme was stable at a pH range 2.6−6.0 and had an optimum pH at 4.0. The enzyme was stable up to 55 °C with an optimum activity at 35 °C. ISSN : 0888-5885 En ligne : http://pubs.acs.org/doi/abs/10.1021/ie801997e

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