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General spectroscopic protocol to obtain the concentration of the superoxide anion radical / Rui-heng Liu in Industrial & engineering chemistry research, Vol. 48 N° 20 (Octobre 2009) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 48 N° 20 (Octobre 2009) . - pp. 9331–9334 Titre : General spectroscopic protocol to obtain the concentration of the superoxide anion radical Type de document : texte imprimé Auteurs : Rui-heng Liu, Auteur ; Shi-yu Fu, Auteur ; Huai-yu Zhan, Auteur Année de publication : 2010 Article en page(s) : pp. 9331–9334 Note générale : Chemical engineering Langues : Anglais (eng) Mots-clés : Nitro  Blue  Tetrazolium  Superoxide  anion  radical  Spectroscopy Résumé : Nitro Blue Tetrazolium (NBT) is a widely used chemical for the determination of superoxide anion radical (O2−) and superoxide dismutase activity in many fundamental biological processes. Its reduction reaction with superoxide and the associated spectral absorption data of its reduction products, i.e., monoformazan (MF) and diformazan (DF), were studied in the current research. The molar extinction coefficient, ε, of each of these two reduction products in dimethyl sulfoxide (DMSO) were calculated to be 120 000 and 235 000 M−1 cm−1, respectively. We propose quantifying these species as a means of obtaining superoxide anion radical concentration or as a general screening protocol for the efficacy of an antioxidant. En ligne : http://pubs.acs.org/doi/abs/10.1021/ie9007826 [article] General spectroscopic protocol to obtain the concentration of the superoxide anion radical [texte imprimé] / Rui-heng Liu, Auteur ; Shi-yu Fu, Auteur ; Huai-yu Zhan, Auteur . - 2010 . - pp. 9331–9334. Chemical engineering Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 48 N° 20 (Octobre 2009) . - pp. 9331–9334 Mots-clés : Nitro  Blue  Tetrazolium  Superoxide  anion  radical  Spectroscopy Résumé : Nitro Blue Tetrazolium (NBT) is a widely used chemical for the determination of superoxide anion radical (O2−) and superoxide dismutase activity in many fundamental biological processes. Its reduction reaction with superoxide and the associated spectral absorption data of its reduction products, i.e., monoformazan (MF) and diformazan (DF), were studied in the current research. The molar extinction coefficient, ε, of each of these two reduction products in dimethyl sulfoxide (DMSO) were calculated to be 120 000 and 235 000 M−1 cm−1, respectively. We propose quantifying these species as a means of obtaining superoxide anion radical concentration or as a general screening protocol for the efficacy of an antioxidant. En ligne : http://pubs.acs.org/doi/abs/10.1021/ie9007826