Comparative morphological, biochemical and immunological studies on two strains of vibrio anguillarum pathogenic in Salmo trutta / Said Dermime (1992) 

Public ISBD Titre : Comparative morphological, biochemical and immunological studies on two strains of vibrio anguillarum pathogenic in Salmo trutta Type de document : texte imprimé Auteurs : Said Dermime, Auteur ; J. B. Alexander, Auteur Editeur : University of Salford Année de publication : 1992 Importance : 269 f. Présentation : ill. Format : 30 cm. Note générale : Thèse de Doctorat: Biologie : United Kingdom, University of Salford : 1992 Bibliogr. f. 270 - 347 Langues : Anglais (eng) Mots-clés : Comparative morphological Biochemical Immunological studies on two strains Vibrio anguillarum pathogenic Salmo trutta Fish immunopathology Index. décimale : D001192 Résumé : Two strains of vibrio anguillarum NCMB571 serotype 01 and NCMB829 serotype 02 were studied. Strain NCMB829 grew more vigorously than NCMB571. Bth weregram-negative rods with a single flagellum. Most NCMB829 cells were thin straight rods whilst NCMB571 were thick and curved. They were obligate, slight halophiles; true lactose fermenters and very sensitive to O/129. They both metabolised glucose and sucrose and NCMB571 also fermented arabinose. Arginine but not ornithine or lysine was metabolised by both strains. The serotypic differences of the two strains were confirmed and were due to the O-specific polysaccharide. A capsule was present in NCMB571 but the results for NCMB829 were equivocal. The antigenic components were constituents of the cell envelopes. When these were separated, the hetero-immunogenicity between the strains was found to be due to O-specific polysaccharides with different molecular weights. Cross-reactivity between the strains was due to common antigenic determinants present in a 47kDa polypeptide in NCMB829 and 51.9kDa in NCMB571. The primary humoral immune response of Salmo trutta to formalin-killed and heat-killed NCMB829 AND NCMB571 was measured by indirect ELISA. With both strains, formalin-killed bacteria stimulated more antibody production than heat-killed ones. The efficacy of the bacterial antigens and the possibility of cross-protection and antigenic competition were studied in vaccinated brown trout. The homologous monovalent and the bivalent bacterins were highly effective in protecting fish from vibriosis. However, the cross-protection between the groups of fish that received heterologous monovalent bacterin was low. Antigenic competition between the strains was not observed. A secondary humoral immune reponse in brown trout after vaccination with bivalent bacterin and challenged with live bacteria was demonstrated and some correlation between antibody production and protection was observed Comparative morphological, biochemical and immunological studies on two strains of vibrio anguillarum pathogenic in Salmo trutta [texte imprimé] / Said Dermime, Auteur ; J. B. Alexander, Auteur . - University of Salford, 1992 . - 269 f. : ill. ; 30 cm. Thèse de Doctorat: Biologie : United Kingdom, University of Salford : 1992 Bibliogr. f. 270 - 347 Langues : Anglais (eng) Mots-clés : Comparative morphological Biochemical Immunological studies on two strains Vibrio anguillarum pathogenic Salmo trutta Fish immunopathology Index. décimale : D001192 Résumé : Two strains of vibrio anguillarum NCMB571 serotype 01 and NCMB829 serotype 02 were studied. Strain NCMB829 grew more vigorously than NCMB571. Bth weregram-negative rods with a single flagellum. Most NCMB829 cells were thin straight rods whilst NCMB571 were thick and curved. They were obligate, slight halophiles; true lactose fermenters and very sensitive to O/129. They both metabolised glucose and sucrose and NCMB571 also fermented arabinose. Arginine but not ornithine or lysine was metabolised by both strains. The serotypic differences of the two strains were confirmed and were due to the O-specific polysaccharide. A capsule was present in NCMB571 but the results for NCMB829 were equivocal. The antigenic components were constituents of the cell envelopes. When these were separated, the hetero-immunogenicity between the strains was found to be due to O-specific polysaccharides with different molecular weights. Cross-reactivity between the strains was due to common antigenic determinants present in a 47kDa polypeptide in NCMB829 and 51.9kDa in NCMB571. The primary humoral immune response of Salmo trutta to formalin-killed and heat-killed NCMB829 AND NCMB571 was measured by indirect ELISA. With both strains, formalin-killed bacteria stimulated more antibody production than heat-killed ones. The efficacy of the bacterial antigens and the possibility of cross-protection and antigenic competition were studied in vaccinated brown trout. The homologous monovalent and the bivalent bacterins were highly effective in protecting fish from vibriosis. However, the cross-protection between the groups of fish that received heterologous monovalent bacterin was low. Antigenic competition between the strains was not observed. A secondary humoral immune reponse in brown trout after vaccination with bivalent bacterin and challenged with live bacteria was demonstrated and some correlation between antibody production and protection was observed

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