| Titre : | Microbial responses to surfactants | | Type de document : | texte imprimé | | Auteurs : | Leila Laouar, Auteur ; K. C. Lowe, Directeur de thèse ; B. J. Mulligan, Directeur de thèse | | Editeur : | Nottingham : University of Nottingham | | Année de publication : | 1993 | | Importance : | 186 f. | | Présentation : | ill. | | Format : | 30 cm. | | Note générale : | Thèse de Doctorat : Génie Chimique : Angleterre, University of Nottingham : 1993
Annexe f. 187 - 226 . Bibliogr. f. 227 - 248 | | Langues : | Anglais (eng) | | Mots-clés : | Pluronic F-68
Microbial cells
Saccharomyces cerevisiae
Triton X-100
Surfactants
Alcohol dehydrogenase | | Index. décimale : | D000893 | | Résumé : | The effects of commercial grade pluronic F-68 or its purified fraction or Triton X-100 on growth of microbial cells in culture have been studied.
Growth of Saccharomyces cerevisiae, as determined by viable cell counts, was unaffected by culture with 0.1 - 10.0% (W/V) of commercial grade Pluronic F-68 or its purified fraction.
However, cultures treated with 10.0% (W/V) commercial grade or purified Pluronic F-68 showed significabtly reduced absorbance up to the onset of the stationary phase of growth, as compared to controls.
The responses to Pluronic F-68 varied according to concentration, age and purity of surfactant.
Additionally, the storage temperature influenced the properties of Pluronic F-68.
Growth in the presence of up to 10.0% (W/V) commercial or purified Pluronic F-68 had no effect on cell-associated protein concentration.
However, a slight, but reproducible, increase in protein concentration was observed during the exponential/stationary phase in cultures supplemented with 0.1 - 1.0% Pluronic F-68.
In addition, whole cell polypeptide profiles were unaffected by the presence of pluronic F-68.
Triton X-100 altered growth kinetics, as reflected by an extended stationary phase and a slowing of the senescence phase.
Additionally, in cultures supplemented with Triton, cell-associated protein concentration was proportional to cellular growth.
Culture in the presence of Triton X-100 did not alter the polypeptide profiles characteristic of untreated cells at different phases of growth.
The application of surfactants for cell permeabilization was also studied using both Pluronic F-68 and Triton X-100, together with Pluronic F-38 and pluronic L-35.
Permeabilization was assayed by direct measurement of alcohol dehydrogenase (ADH) activity in whole cells.
Mean ADH activity following incubation of S. cerevisiae with 0.2% (W/V or V/V) of either commercial or purified Pluronic F-68, Pluronic F-38, Pluronic L-35 or Triton X-100 for 30 min at 20°C was significantly greater than when cells were exposed to an identical concentration of the previously studied permeabilizing agent, cetyltrimethylammonium bromide (CTAB). |
Microbial responses to surfactants [texte imprimé] / Leila Laouar, Auteur ; K. C. Lowe, Directeur de thèse ; B. J. Mulligan, Directeur de thèse . - Nottingham : University of Nottingham, 1993 . - 186 f. : ill. ; 30 cm. Thèse de Doctorat : Génie Chimique : Angleterre, University of Nottingham : 1993
Annexe f. 187 - 226 . Bibliogr. f. 227 - 248 Langues : Anglais ( eng) | Mots-clés : | Pluronic F-68
Microbial cells
Saccharomyces cerevisiae
Triton X-100
Surfactants
Alcohol dehydrogenase | | Index. décimale : | D000893 | | Résumé : | The effects of commercial grade pluronic F-68 or its purified fraction or Triton X-100 on growth of microbial cells in culture have been studied.
Growth of Saccharomyces cerevisiae, as determined by viable cell counts, was unaffected by culture with 0.1 - 10.0% (W/V) of commercial grade Pluronic F-68 or its purified fraction.
However, cultures treated with 10.0% (W/V) commercial grade or purified Pluronic F-68 showed significabtly reduced absorbance up to the onset of the stationary phase of growth, as compared to controls.
The responses to Pluronic F-68 varied according to concentration, age and purity of surfactant.
Additionally, the storage temperature influenced the properties of Pluronic F-68.
Growth in the presence of up to 10.0% (W/V) commercial or purified Pluronic F-68 had no effect on cell-associated protein concentration.
However, a slight, but reproducible, increase in protein concentration was observed during the exponential/stationary phase in cultures supplemented with 0.1 - 1.0% Pluronic F-68.
In addition, whole cell polypeptide profiles were unaffected by the presence of pluronic F-68.
Triton X-100 altered growth kinetics, as reflected by an extended stationary phase and a slowing of the senescence phase.
Additionally, in cultures supplemented with Triton, cell-associated protein concentration was proportional to cellular growth.
Culture in the presence of Triton X-100 did not alter the polypeptide profiles characteristic of untreated cells at different phases of growth.
The application of surfactants for cell permeabilization was also studied using both Pluronic F-68 and Triton X-100, together with Pluronic F-38 and pluronic L-35.
Permeabilization was assayed by direct measurement of alcohol dehydrogenase (ADH) activity in whole cells.
Mean ADH activity following incubation of S. cerevisiae with 0.2% (W/V or V/V) of either commercial or purified Pluronic F-68, Pluronic F-38, Pluronic L-35 or Triton X-100 for 30 min at 20°C was significantly greater than when cells were exposed to an identical concentration of the previously studied permeabilizing agent, cetyltrimethylammonium bromide (CTAB). |
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