Investigation of the Porosity Variation during Chromatographic Experiments / Nicola Forrer in Industrial & engineering chemistry research, Vol. 47 N° 23 (Décembre 2008)  

Public ISBD [article]  inIndustrial & engineering chemistry research > Vol. 47 N° 23 (Décembre 2008) . - p. 9133–9140 Titre : Investigation of the Porosity Variation during Chromatographic Experiments Type de document : texte imprimé Auteurs : Nicola Forrer, Auteur ; Olga Kartachova, Auteur ; Alessandro Butté, Auteur Année de publication : 2009 Article en page(s) : p. 9133–9140 Note générale : Chemistry engineering Langues : Anglais (eng) Mots-clés : Investigation of the Porosity Variation during Chromatographic Experiments Résumé : Poly(vinyl pyrrolidone) tracers are used to investigate the porosity variation on ion-exchange materials. The porosity was measured as a function of the salt concentration in the mobile phase and of the amount of protein adsorbed on the material. Immunoglobulin G (IgG) and human serum albumin (HSA) are selected as proteins. Two materials with different pore size distributions were investigated. The surface of both resins is functionalized with tentacles that carry a terminal ionic group. In both materials, a small increase of the accessible porosity was observed for increasing salt concentrations. A larger effect was observed in the presence of protein adsorption. The accessible porosity decreases quickly as the amount of adsorbed proteins increases. The decrease in porosity due to the HSA adsorption is smaller than the decrease caused by IgG. En ligne : http://pubs.acs.org/doi/abs/10.1021/ie800131t [article] Investigation of the Porosity Variation during Chromatographic Experiments [texte imprimé] / Nicola Forrer, Auteur ; Olga Kartachova, Auteur ; Alessandro Butté, Auteur . - 2009 . - p. 9133–9140. Chemistry engineering Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 47 N° 23 (Décembre 2008) . - p. 9133–9140 Mots-clés : Investigation of the Porosity Variation during Chromatographic Experiments Résumé : Poly(vinyl pyrrolidone) tracers are used to investigate the porosity variation on ion-exchange materials. The porosity was measured as a function of the salt concentration in the mobile phase and of the amount of protein adsorbed on the material. Immunoglobulin G (IgG) and human serum albumin (HSA) are selected as proteins. Two materials with different pore size distributions were investigated. The surface of both resins is functionalized with tentacles that carry a terminal ionic group. In both materials, a small increase of the accessible porosity was observed for increasing salt concentrations. A larger effect was observed in the presence of protein adsorption. The accessible porosity decreases quickly as the amount of adsorbed proteins increases. The decrease in porosity due to the HSA adsorption is smaller than the decrease caused by IgG. En ligne : http://pubs.acs.org/doi/abs/10.1021/ie800131t

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