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Preparation and evaluation of cellulose adsorbents for hydrophobic charge induction chromatography / Hai-Feng Xia in Industrial & engineering chemistry research, Vol. 47 N° 23 (Décembre 2008) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 47 N° 23 (Décembre 2008) . - p. 9566–9572 Titre : Preparation and evaluation of cellulose adsorbents for hydrophobic charge induction chromatography Type de document : texte imprimé Auteurs : Hai-Feng Xia, Auteur ; Dong-Qiang Lin, Auteur ; Li-Ping Wang, Auteur Année de publication : 2009 Article en page(s) : p. 9566–9572 Note générale : Chemistry engineering Langues : Anglais (eng) Mots-clés : Evaluation  of  cellulose  adsorbents  Charge  induction  chromatography Résumé : Hydrophobic charge induction chromatography (HCIC) has been proven to be an efficient technique for antibody purification. Several HCIC adsorbents were prepared with macroporous cellulose−tungsten carbide composite beads (Cell-TuC) as the matrix. First, the cellulose beads were activated by allyl bromide (AB) or divinyl sulfone (DVS), and then they were coupled with three types of mercaptoheterocyclic groups—4-mercapto-ethyl-pyridine hydrochloride (MEP), 2-mercapto-1-methyl-imidazole (MMI), and 2-mercapto-benzimidazole (MBI)—as the HCIC ligands. Four types of HCIC adsorbents were obtained, labeled Cell-TuC-AB-MEP, Cell-TuC-DVS-MEP, Cell-TuC-DVS-MMI, and Cell-TuC-DVS-MBI. The activation and coupling conditions were optimized for high ligand density. The isotherm adsorption of immunoglobulin of egg yolk (IgY) on four HCIC adsorbents were investigated. High adsorption capacities of IgY could be obtained for all four adsorbents at pH 7, and low adsorption of IgY at pH 4 and of bovine serum albumin (BSA) at pH 7 was observed, which indicates that the HCIC adsorbents prepared have a potential application for antibody purification. En ligne : http://pubs.acs.org/doi/abs/10.1021/ie800662r [article] Preparation and evaluation of cellulose adsorbents for hydrophobic charge induction chromatography [texte imprimé] / Hai-Feng Xia, Auteur ; Dong-Qiang Lin, Auteur ; Li-Ping Wang, Auteur . - 2009 . - p. 9566–9572. Chemistry engineering Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 47 N° 23 (Décembre 2008) . - p. 9566–9572 Mots-clés : Evaluation  of  cellulose  adsorbents  Charge  induction  chromatography Résumé : Hydrophobic charge induction chromatography (HCIC) has been proven to be an efficient technique for antibody purification. Several HCIC adsorbents were prepared with macroporous cellulose−tungsten carbide composite beads (Cell-TuC) as the matrix. First, the cellulose beads were activated by allyl bromide (AB) or divinyl sulfone (DVS), and then they were coupled with three types of mercaptoheterocyclic groups—4-mercapto-ethyl-pyridine hydrochloride (MEP), 2-mercapto-1-methyl-imidazole (MMI), and 2-mercapto-benzimidazole (MBI)—as the HCIC ligands. Four types of HCIC adsorbents were obtained, labeled Cell-TuC-AB-MEP, Cell-TuC-DVS-MEP, Cell-TuC-DVS-MMI, and Cell-TuC-DVS-MBI. The activation and coupling conditions were optimized for high ligand density. The isotherm adsorption of immunoglobulin of egg yolk (IgY) on four HCIC adsorbents were investigated. High adsorption capacities of IgY could be obtained for all four adsorbents at pH 7, and low adsorption of IgY at pH 4 and of bovine serum albumin (BSA) at pH 7 was observed, which indicates that the HCIC adsorbents prepared have a potential application for antibody purification. En ligne : http://pubs.acs.org/doi/abs/10.1021/ie800662r