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Response surface methodology for optimization of lipase production by an immobilized newly isolated penicillium sp. / Elisangela Wolski ; Elisangele Menusi ; Marcio Mazutti in Industrial & engineering chemistry research, Vol. 47 N° 23 (Décembre 2008) 

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 47 N° 23 (Décembre 2008) . - p. 9651–9657 Titre : Response surface methodology for optimization of lipase production by an immobilized newly isolated penicillium sp. Type de document : texte imprimé Auteurs : Elisangela Wolski, Auteur ; Elisangele Menusi, Auteur ; Marcio Mazutti, Auteur Année de publication : 2009 Article en page(s) : p. 9651–9657 Note générale : Chemistry engineering Langues : Anglais (eng) Mots-clés : Surface  methodology  Lipase  production  Penicillium  sp Résumé : This work reports the use of response surface methodology to optimize the lipase production by submerged fermentation using immobilized cells of a newly isolated Penicillium sp. For this, experimental designs were employed to evaluate the best condition for microorganism immobilization. The optimum experimental conditions for immobilized microorganisms were determined as 2.5% (w/v) of calcium alginate, 0.2% (w/v) of CaCl2, and 1.5% (v/v) of glutaraldehyde and 6 h of curing time, yielding a lipase activity as high as 20.96 U/mL in 120 h of fermentation. The activity obtained is higher than those observed with free cells of this microorganism and comparable to results presented in the literature for lipase production with immobilized cells from several microorganisms. The crude enzymatic extract presented optimum pH and temperature of 7.0 and 37 °C, respectively, and maintained its activity for 60 days storage at −10 °C. En ligne : http://pubs.acs.org/doi/abs/10.1021/ie800658j [article] Response surface methodology for optimization of lipase production by an immobilized newly isolated penicillium sp. [texte imprimé] / Elisangela Wolski, Auteur ; Elisangele Menusi, Auteur ; Marcio Mazutti, Auteur . - 2009 . - p. 9651–9657. Chemistry engineering Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 47 N° 23 (Décembre 2008) . - p. 9651–9657 Mots-clés : Surface  methodology  Lipase  production  Penicillium  sp Résumé : This work reports the use of response surface methodology to optimize the lipase production by submerged fermentation using immobilized cells of a newly isolated Penicillium sp. For this, experimental designs were employed to evaluate the best condition for microorganism immobilization. The optimum experimental conditions for immobilized microorganisms were determined as 2.5% (w/v) of calcium alginate, 0.2% (w/v) of CaCl2, and 1.5% (v/v) of glutaraldehyde and 6 h of curing time, yielding a lipase activity as high as 20.96 U/mL in 120 h of fermentation. The activity obtained is higher than those observed with free cells of this microorganism and comparable to results presented in the literature for lipase production with immobilized cells from several microorganisms. The crude enzymatic extract presented optimum pH and temperature of 7.0 and 37 °C, respectively, and maintained its activity for 60 days storage at −10 °C. En ligne : http://pubs.acs.org/doi/abs/10.1021/ie800658j