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PH-sensitive magnetic ion exchanger for protein separation / Liangrong Yang in Industrial & engineering chemistry research, Vol. 48 N°2 (Janvier 2009)

Public ISBD [article]  in Industrial & engineering chemistry research > Vol. 48 N°2 (Janvier 2009) . - p. 944–950 Titre : PH-sensitive magnetic ion exchanger for protein separation Type de document : texte imprimé Auteurs : Liangrong Yang, Auteur ; Chen, Guo, Auteur ; Shu Chen, Auteur ; Feng Wang, Auteur ; Jing Wang, Auteur ; Zhentao An, Auteur Année de publication : 2009 Article en page(s) : p. 944–950 Note générale : chimical engineering Langues : Anglais (eng) Mots-clés : pH-sensitive Résumé : A pH-sensitive magnetic ion exchanger was synthesized by binding carboxymethylated chitosan (CMCH) covalently on the surface of Fe3O4 nanoparticles. The diameter for magnetic particles observed at 25 °C was 15 nm. The ion exchanger was superparamagnetic with a saturation magnetization of 64.21 emu/g and an isoelectric point (pI) of 5.75. In a model system, the laccase adsorption capacity reached equilibrium within 15 min (pH 5). The adsorption process followed the Langmuir adsorption isotherm, and the maximum equilibrium adsorption capacity was calculated to be 198.81 mg/g. The laccase can be completely desorbed at pH 8. About 97% laccase can be effectively desorbed from the surface of particles within 15 min. Moreover, the specific activity of the laccase remained constant during the adsorption and desorption process. Finally, the pH-sensitive magnetic ion exchanger was used for separation of laccase directly from culture supernatant, and nearly pure laccase was isolated by a single step with an activity recovery rate of 63%. [article] PH-sensitive magnetic ion exchanger for protein separation [texte imprimé] / Liangrong Yang, Auteur ; Chen, Guo, Auteur ; Shu Chen, Auteur ; Feng Wang, Auteur ; Jing Wang, Auteur ; Zhentao An, Auteur . - 2009 . - p. 944–950. chimical engineering Langues : Anglais (eng) in Industrial & engineering chemistry research > Vol. 48 N°2 (Janvier 2009) . - p. 944–950 Mots-clés : pH-sensitive Résumé : A pH-sensitive magnetic ion exchanger was synthesized by binding carboxymethylated chitosan (CMCH) covalently on the surface of Fe3O4 nanoparticles. The diameter for magnetic particles observed at 25 °C was 15 nm. The ion exchanger was superparamagnetic with a saturation magnetization of 64.21 emu/g and an isoelectric point (pI) of 5.75. In a model system, the laccase adsorption capacity reached equilibrium within 15 min (pH 5). The adsorption process followed the Langmuir adsorption isotherm, and the maximum equilibrium adsorption capacity was calculated to be 198.81 mg/g. The laccase can be completely desorbed at pH 8. About 97% laccase can be effectively desorbed from the surface of particles within 15 min. Moreover, the specific activity of the laccase remained constant during the adsorption and desorption process. Finally, the pH-sensitive magnetic ion exchanger was used for separation of laccase directly from culture supernatant, and nearly pure laccase was isolated by a single step with an activity recovery rate of 63%.